asebohydro.blogg.se

Illumina raw geneious tutorial
Illumina raw geneious tutorial








  1. #Illumina raw geneious tutorial how to#
  2. #Illumina raw geneious tutorial archive#

Genome Lytic phage pPM_01 Proteus mirabilis Urinary tract infection. Researchers can easily query, manage, share, and analyze all of their sequence data intuitively within a user-friendly interface. The inability to lysogenize a host, the absence of endotoxin genes in the annotated genome, and the lytic behavior suggest phage pPM_01 as a possible safe biological candidate to control P. Geneious R10 provides a solution to these problems by incorporating industry-leading algorithms and applications for genomic & protein sequence analyses via a single robust desktop program. Host range determination and SDS-PAGE analysis were also performed. The whole genome sequence showed a low similarity to known phages (i.e., Enterobacter phage Enc34 and Enterobacteria phage Chi). Functional clusters of related potential genes were defined (structural, lytic, packaging, replication, modification, and modulatory). Seventy putative genes were identified and annotated using various bioinformatics tools including RAST, Geneious v.R7, National Center for Biotechnology Information (NCBI) BLAST, and tRNAscan-SE-v1.3 Search. The whole genome contains a total of 58,546 bp of linear double-stranded DNA with a G+C content of 46.9%. Revisions or updates to GenBank entries can be made by the submitters at any time.

#Illumina raw geneious tutorial archive#

The genome was sequenced using Illumina technology and the reads obtained were assembled using CLC Genomic Workbench v.7.0.3. Runs of next-generation sequencing, for example from 454 or Illumina, can be submitted to the Sequence Read Archive (SRA). mirabilis), with its complete genome sequence successfully determined. Transmission electron microscopy revealed phage pPM_01 to be a siphovirus (the first reported virus to infect P. In this paper, we characterize the biology and complete genome sequence of the phage.

#Illumina raw geneious tutorial how to#

So this tutorial is about how to clean/trim/edit raw DNA sequences using Geneious. Phage pPM_01 was previously isolated from a raw sewage treatment facility located in Batu Maung, Penang, Malaysia, and it was highly lytic against Proteus mirabilis, which causes urinary tract infections in humans. AoA everyone I hope you all are doing fine.










Illumina raw geneious tutorial